Hypoxia adipose stem cell-derived exosomes promote high-quality healing of diabetic wound involves activation of PI3K/Akt pathways

Abstract Refractory diabetic wounds can cause persistent inflammation and delayed healing due to hypoxia. Currently, no optimal solution is available. Exosomes of adipose stem cells (ADSCs-exo) may promote skin wound healing, however, molecular mechanisms remains mysterious. We found significantly enhanced survival proliferation after hypoxia induction compared normoxia. Here, we aimed investigate if hypoxic exosomes (HypADSCs-exo) participate in adaptability accelerate healing. Based on high-throughput sequencing, 215 microRNAs (miRNAs) were upregulated 369 miRNAs downregulated HypADSCs-exo ADSCs-exo. Up-regulated miR-21-3p, miR-126-5p, miR-31-5p whereas down-regulated gene miR-99b miR-146-a correlated with According Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG), might regulate cell metabolism, differentiation Transforming growth factor-? (TGF-?) function. Consistently, HpyADSCs-exo could inhibit through PI3K/AKT signaling pathway. Collectively, as an alternative strategy improve